8Y6B

Structure of human LGI1-ADAM22 complex in space group P212121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97800
Spacegroup name	P 21 21 21
Unit cell lengths	55.448, 196.078, 420.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.460 - 3.490
R-factor	0.238
Rwork	0.236
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w8a 5sxm 3g5c
RMSD bond length	0.003
RMSD bond angle	0.832
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.560
High resolution limit [Å]	3.490	3.490
Rmerge	0.108	0.540
Rpim	0.064
Number of reflections	58327	2737
<I/σ(I)>	14.582	2.806
Completeness [%]	97.0	94.3
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.8M sodium/potassium tartrate, 0.1M Tris pH=7.0, 0.2M lithium sulfate.