8Y0D
Crystal structure of SauCas9 in complex with sgRNA and 20nt ssDNA target
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 68.962, 145.567, 97.528 |
| Unit cell angles | 90.00, 93.99, 90.00 |
Refinement procedure
| Resolution | 48.650 - 3.920 |
| R-factor | 0.289 |
| Rwork | 0.286 |
| R-free | 0.31760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5axw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.802 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.970 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rpim | 0.063 | 0.432 |
| Number of reflections | 17461 | 827 |
| <I/σ(I)> | 9.67 | |
| Completeness [%] | 98.9 | 97.3 |
| Redundancy | 4.5 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Sodium chloride, 0.1 M Sodium HEPES, pH 7.0, 11% PEG 8000 |
