8Y0D
Crystal structure of SauCas9 in complex with sgRNA and 20nt ssDNA target
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (B) | RNA (73-MER) | polymer | 74 | 23635.0 | 1 | Staphylococcus aureus | ||
| 2 | B (C) | DNA (5'-D(P*GP*TP*AP*AP*AP*GP*TP*TP*AP*AP*AP*TP*AP*GP*CP*AP*GP*AP*C)-3') | polymer | 20 | 6175.0 | 1 | Staphylococcus aureus | ||
| 3 | C (A) | CRISPR-associated endonuclease Cas9 | polymer | 1053 | 124083.6 | 1 | UniProt (J7RUA5) Pfam (PF13395) Pfam (PF22702) Pfam (PF18061) Pfam (PF18070) Pfam (PF21574) | Staphylococcus aureus | SaCas9 |
| 4 | D, E (B, A) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
A: 1 - 1053 (UniProt: J7RUA5)
| PDB | External Database | Details |
|---|---|---|
| Ala 580 | Asn 580 | conflict |
| Ala 946 | Cys 946 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 153893.7 | |
| All* | Total formula weight | 153893.7 |
