8Y08
Crystal structure of LbCas12a in complex with crRNA and 14nt target DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 121.390, 142.292, 208.582 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.070 - 3.640 |
| R-factor | 0.2532 |
| Rwork | 0.252 |
| R-free | 0.28490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xuz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.684 |
| Data reduction software | HKL-3000 (7.21) |
| Data scaling software | HKL-3000 (7.21) |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.710 |
| High resolution limit [Å] | 3.640 | 3.640 |
| Rpim | 0.115 | 0.366 |
| Number of reflections | 39629 | 1938 |
| <I/σ(I)> | 6.75 | |
| Completeness [%] | 95.9 | 96 |
| Redundancy | 9.4 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Li2SO4, 30 mM Na2HPO4, 43.75 mM citric acid, 13% PEG 1000 |
