8Y08

Crystal structure of LbCas12a in complex with crRNA and 14nt target DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	LbCas12a	polymer	1228	143888.4	2	UniProt (A0A5S8WF58) Pfam (PF18501) Pfam (PF21918) Pfam (PF22222) Pfam (PF18516) Pfam (PF18510) UniProt (by SIFTS) (A0A182DWE3)	Lachnospiraceae bacterium ND2006
2	B, F (B, F)	RNA (35-MER)	polymer	40	12804.7	2		Lachnospiraceae bacterium ND2006
3	C, G (C, G)	DNA (5'-D(P*CP*TP*TP*TP*AP*CP*TP*GP*GP*AP*TP*GP*CP*GP*TP*AP*AP*AP*GP*GP*AP*CP*G)-3')	polymer	23	7120.6	2		Lachnospiraceae bacterium ND2006
4	D, H (D, H)	DNA (5'-D(*CP*GP*TP*CP*CP*TP*TP*TP*AP*TP*T)-3')	polymer	11	3290.2	2		Lachnospiraceae bacterium ND2006
5	I, J, K (A, B)	LITHIUM ION	non-polymer		6.9	3	Chemie (LI)
6	L (E)	water	water		18.0	1	Chemie (HOH)

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	334207.6
Non-Polymers*	Number of molecules	3
	Total formula weight	20.8
All*	Total formula weight	334228.4

*Water molecules are not included.