8WV1
Ambient Temperature Structure of 50S Ribosomal Subunit from Thermus Thermophilus
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SLAC LCLS BEAMLINE CXI |
| Synchrotron site | SLAC LCLS |
| Beamline | CXI |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2016-07-09 |
| Detector | CS-PAD CXI-1 |
| Wavelength(s) | 1.29 |
| Spacegroup name | P 41 |
| Unit cell lengths | 303.800, 303.800, 434.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.996 - 3.990 |
| R-factor | 0.2961 |
| Rwork | 0.296 |
| R-free | 0.36170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.521 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.996 | 4.100 |
| High resolution limit [Å] | 3.990 | 3.990 |
| Number of reflections | 567145 | 56714 |
| <I/σ(I)> | 3.85 | |
| Completeness [%] | 86.1 | |
| Redundancy | 5.099 | |
| CC(1/2) | 0.800 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20% w/v PEG 3350 200 mM ammonium sulfate tribasic |
