8WOW
Crystal structure of Arabidopsis thaliana UDP-glucose 4-epimerase 2 (AtUGE2) complexed with UDP, I160L/G233A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-11 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.99988 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.228, 108.929, 130.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.110 - 2.600 |
R-factor | 0.17582 |
Rwork | 0.172 |
R-free | 0.24423 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ek6 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.557 |
Data reduction software | XDS (Feb 5, 2021) |
Data scaling software | XDS (Feb 5, 2021) |
Phasing software | MOLREP (11.0) |
Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.207 | 0.633 |
Rpim | 0.085 | 0.257 |
Number of reflections | 22589 | 2701 |
<I/σ(I)> | 5.9 | 3.4 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.8 | 7 |
CC(1/2) | 0.976 | 0.852 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 20-22% (w/v) PEG3350 and 0.16-0.18 M magnesium formate |