8WOW

Crystal structure of Arabidopsis thaliana UDP-glucose 4-epimerase 2 (AtUGE2) complexed with UDP, I160L/G233A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	UDP-glucose 4-epimerase 2	polymer	370	40612.8	2	UniProt (Q9T0A7) Pfam (PF16363) In PDB	Arabidopsis thaliana (thale cress)
2	A, B	URIDINE-5'-DIPHOSPHATE	non-polymer		404.2	2	Chemie (UDP)
3	A, B	NICOTINAMIDE-ADENINE-DINUCLEOTIDE	non-polymer		663.4	2	Chemie (NAD)
4	B	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5		water	water		18.0	62	Chemie (HOH)

Sequence modifications

A, B: 1 - 350 (UniProt: Q9T0A7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Leu 160	Ile 160	engineered mutation
Ala 233	Gly 233	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81225.5
Non-Polymers*	Number of molecules	5
	Total formula weight	2231.2
All*	Total formula weight	83456.8

*Water molecules are not included.