8W6P

Crystal structure of dimeric murine SMPDL3A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-16
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	122.225, 122.225, 79.557
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.270 - 1.910
R-factor	0.2182
Rwork	0.218
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.261
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.270	1.940
High resolution limit [Å]	1.910	1.910
Rmerge	0.101	0.768
Number of reflections	89995	3909
<I/σ(I)>	17.9	1
Completeness [%]	98.6	85.1
Redundancy	7.5	3.5
CC(1/2)	0.999	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	0.1 M sodium citrate [pH 5.5], 0.2M ammonium sulfate and 30% PEG 4000