8W0V
Crystal structure of broadly neutralizing antibody hcab55 in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2021-06-29 |
Detector | DECTRIS PILATUS3 X 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.019, 88.840, 175.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.670 - 2.590 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.25740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.109 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 88.840 | 2.710 |
High resolution limit [Å] | 2.590 | 2.590 |
Rmerge | 0.166 | 1.210 |
Rmeas | 0.183 | 1.335 |
Rpim | 0.076 | 0.554 |
Total number of observations | 183914 | 22282 |
Number of reflections | 32994 | 3974 |
<I/σ(I)> | 6.8 | 1.7 |
Completeness [%] | 99.0 | |
Redundancy | 5.6 | 5.6 |
CC(1/2) | 0.982 | 0.595 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 298 | 0.2 M lithium citrate tribasic tetrahydrate and 20% PEG 3,350 |