8VTV

Crystal structure of the wild-type Thermus thermophilus 70S ribosome in complex with macrolone MCX-91, mRNA, aminoacylated A-site Phe-tRNAphe, aminoacylated P-site fMet-tRNAmet, and deacylated E-site tRNAphe at 2.55A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-24
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	211.850, 451.310, 630.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.660 - 2.550
R-factor	0.2217
Rwork	0.220
R-free	0.26160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhw
RMSD bond length	0.004
RMSD bond angle	0.839
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.660	122.660	2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.193	0.052	1.818
Rmeas	0.215	0.058	2.029
Total number of observations	9557286
Number of reflections	1921762	22148	141597
<I/σ(I)>	7.04	21.13	0.98
Completeness [%]	99.4	97.1	99.4
Redundancy	4.973	5.365	4.825
CC(1/2)	0.991	0.995	0.199

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME