6XHW

Crystal structure of the A2058-unmethylated Thermus thermophilus 70S ribosome in complex with mRNA, aminoacylated A- and P-site tRNAs, and deacylated E-site tRNA at 2.50A resolution

This is a non-PDB format compatible entry.

Summary for 6XHW

• Entry DOI: 10.2210/pdb6xhw/pdb
• Descriptor: 23S Ribosomal RNA, 50S ribosomal protein L14, 50S ribosomal protein L15, ... (61 entities in total)
• Functional Keywords: macrolide, antibiotic, resistance, methylation, a2058, 23s rrna, 70s ribosome, inhibition of translation, peptidyl transferase center, nascent peptide exit tunnel, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 112
• Total formula weight: 4571007.67

Authors

Svetlov, M.S.,Syroegin, E.A.,Aleksandrova, E.V.,Atkinson, G.C.,Gregory, S.T.,Mankin, A.S.,Polikanov, Y.S. (deposition date: 2020-06-19, release date: 2020-12-23, Last modification date: 2023-11-15)

• Primary citation: Svetlov, M.S.,Syroegin, E.A.,Aleksandrova, E.V.,Atkinson, G.C.,Gregory, S.T.,Mankin, A.S.,Polikanov, Y.S.; Structure of Erm-modified 70S ribosome reveals the mechanism of macrolide resistance.; Nat.Chem.Biol., 17:412-420, 2021; PubMed: 33462493; DOI: 10.1038/s41589-020-00715-0

Experimental method

X-RAY DIFFRACTION (2.5 Å)