8VLB
Crystal structure of EloBC-VHL-CDO1 complex bound to compound 4 molecular glue
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-17 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.440, 178.116, 104.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.220 - 2.900 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.833 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.460 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.171 | 2.397 |
| Number of reflections | 23239 | 3885 |
| <I/σ(I)> | 8.1 | 0.9 |
| Completeness [%] | 95.3 | 100 |
| Redundancy | 6.6 | 6.9 |
| CC(1/2) | 0.996 | 0.411 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2M SODIUM CITRATE, 0.1M BIS-TRIS-PROPANE PH 6.5, 20% PEG3350 |
