8VK9
Structure of UbV.d2.3 in complex with Ube2d2-S22R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.850, 56.314, 117.499 |
| Unit cell angles | 86.48, 78.47, 60.32 |
Refinement procedure
| Resolution | 41.200 - 2.000 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.053 | 0.765 |
| Number of reflections | 80451 | 7996 |
| <I/σ(I)> | 10.2 | 1.2 |
| Completeness [%] | 97.5 | 96.7 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.998 | 0.678 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.1 M Sodium HEPES pH 7.0, 15% w/v PEG 20,000 |
