8VEU

Crystal structure of PRMT5:MEP50 in complex with MTA and oxamide compound 23

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97779
Spacegroup name	I 2 2 2
Unit cell lengths	103.370, 138.790, 179.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.800 - 2.460
R-factor	0.2034
Rwork	0.201
R-free	0.25027
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.006
RMSD bond angle	1.483
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.550
High resolution limit [Å]	2.460	2.460
Rmerge	0.078	0.934
Rmeas	0.085	1.013
Rpim	0.033	0.390
Total number of observations		30741
Number of reflections	47213	4610
<I/σ(I)>	14.5	2.2
Completeness [%]	99.9
Redundancy	6.7	6.7
CC(1/2)	0.999	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	291	10% PEG 4000, 0.2M magnesium chloride, 0.1M sodium citrate (pH 6.0)