Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 137.403, 149.456, 157.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.180 - 3.460 |
| R-factor | 0.2601 |
| Rwork | 0.257 |
| R-free | 0.31510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.580 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmerge | 0.114 | 1.000 |
| Rmeas | 0.119 | 1.000 |
| Rpim | 0.035 | 0.495 |
| Number of reflections | 25000 | 3818 |
| <I/σ(I)> | 11.2 | 0.8 |
| Completeness [%] | 95.8 | 89.9 |
| Redundancy | 9.9 | 6.5 |
| CC(1/2) | 1.000 | 0.706 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 8000, 0.1 M HEPES, pH 6.8 |
