8V07

Crystal structure of mouse PLD3 co-crystallized with 5'Pi-ssDNA for 30 days

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1
Unit cell lengths	54.523, 54.789, 203.574
Unit cell angles	83.65, 89.10, 89.90

Refinement procedure

Resolution	37.120 - 1.990
R-factor	0.2256
Rwork	0.224
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.781
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	1.990	5.430	2.000
Rmerge	0.091	0.045	0.701
Rmeas		0.056	0.919
Rpim		0.032	0.587
Number of reflections	131978	6644	5605
<I/σ(I)>	6.9
Completeness [%]	83.1	83.7	69.3
Redundancy	2.3	2.6	1.9
CC(1/2)		0.994	0.529

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	1.6 M ammonium sulfate, 0.5 mM magnesium chloride, 0.1 M citric acid, pH 4.0