8UWB

Crystal structure of PP2A PPP2R1A-PPP2CA-PPP2R5E phosphatase.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97911
Spacegroup name	P 1
Unit cell lengths	83.839, 92.597, 134.657
Unit cell angles	83.77, 72.46, 73.46

Refinement procedure

Resolution	128.360 - 3.150
R-factor	0.2227
Rwork	0.221
R-free	0.26290
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.504
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	128.400	3.263
High resolution limit [Å]	3.150	3.150
Rmerge	0.154	2.011
Rmeas	0.166	2.177
Rpim	0.060	0.821
Number of reflections	62227	5795
<I/σ(I)>	9.16	0.82
Completeness [%]	96.4	90.61
Redundancy	7.4	0.82
CC(1/2)	0.998	0.397

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.77	297	23% (w/v) PEG 20k, 0.1 M BIS-TRIS pH 5.77