8UWB
Crystal structure of PP2A PPP2R1A-PPP2CA-PPP2R5E phosphatase.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 |
| Unit cell lengths | 83.839, 92.597, 134.657 |
| Unit cell angles | 83.77, 72.46, 73.46 |
Refinement procedure
| Resolution | 128.360 - 3.150 |
| R-factor | 0.2227 |
| Rwork | 0.221 |
| R-free | 0.26290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.504 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.400 | 3.263 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.154 | 2.011 |
| Rmeas | 0.166 | 2.177 |
| Rpim | 0.060 | 0.821 |
| Number of reflections | 62227 | 5795 |
| <I/σ(I)> | 9.16 | 0.82 |
| Completeness [%] | 96.4 | 90.61 |
| Redundancy | 7.4 | 0.82 |
| CC(1/2) | 0.998 | 0.397 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.77 | 297 | 23% (w/v) PEG 20k, 0.1 M BIS-TRIS pH 5.77 |
