8UDC
Crystal structure of TcPINK1 in complex with CYC116
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-21 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 53.021, 53.021, 546.158 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.920 - 3.100 |
| R-factor | 0.2585 |
| Rwork | 0.255 |
| R-free | 0.32930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.558 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.920 | 3.310 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.850 | |
| Number of reflections | 9497 | 1611 |
| <I/σ(I)> | 7.8 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 35.3 | 38.6 |
| CC(1/2) | 0.996 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 90 mM HEPES 7.0, 17.5% PEG 4K, 0.13 M Ammonium sulfate |
