8U15

The ternary complex structure of DDB1-CRBN-SALL4(ZF1,2)-short bound to CC-220

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-26
Detector	ADSC QUANTUM 210
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	110.729, 95.727, 150.947
Unit cell angles	90.00, 91.72, 90.00

Refinement procedure

Resolution	61.480 - 2.950
R-factor	0.2222
Rwork	0.220
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.516
Data reduction software	XDS
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	95.730	3.020
High resolution limit [Å]	2.950	2.950
Rmerge	0.104	2.947
Rmeas	0.339	3.177
Rpim	0.128	1.182
Total number of observations	462088	32233
Number of reflections	66770	4494
<I/σ(I)>	5.1	0.8
Completeness [%]	100.0
Redundancy	6.9	7.2
CC(1/2)	0.986	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M Sodium malonate, 20% PEG 3350