8TVC
Crystal structure of rA3G-ssDNA-AA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.429, 68.461, 126.735 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.080 - 1.930 |
| R-factor | 0.1792 |
| Rwork | 0.178 |
| R-free | 0.20840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.869 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.080 | 1.999 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.078 | 0.501 |
| Rmeas | 0.086 | 0.566 |
| Number of reflections | 36833 | 3472 |
| <I/σ(I)> | 10.8 | 0.88 |
| Completeness [%] | 99.3 | 94.69 |
| Redundancy | 5.9 | 4.5 |
| CC(1/2) | 0.998 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 18% PEG 3350, 0.1 M Bis-Tris Propane pH 7.3, 0.2 M Sodium/Potassium Phosphate |
