8TTA

Structure of retromer VPS29-VPS35 (483-796) complexed with Fam21A repeat 21 (1328-1341)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953700
Spacegroup name	C 1 2 1
Unit cell lengths	123.730, 165.577, 68.000
Unit cell angles	90.00, 117.51, 90.00

Refinement procedure

Resolution	48.750 - 3.460
R-factor	0.223
Rwork	0.221
R-free	0.25250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.075
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.310	3.790
High resolution limit [Å]	3.460	3.460
Rmerge	0.342	1.233
Rmeas	0.370	1.330
Rpim	0.139	0.494
Total number of observations	110783	26173
Number of reflections	15793	3693
<I/σ(I)>	4.5	1.3
Completeness [%]	99.3
Redundancy	7	7.1
CC(1/2)	0.993	0.762

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	0.1 M MES pH 6.5, 0.1 M Magnesium Acetate, 10% PEG10,000