8TS5
Structure of the apo FabS1C_C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.688, 73.792, 174.202 |
| Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
| Resolution | 86.560 - 2.100 |
| R-factor | 0.222 |
| Rwork | 0.220 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8trs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.968 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.560 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.095 | 0.648 |
| Rmeas | 0.114 | 0.768 |
| Rpim | 0.061 | 0.408 |
| Number of reflections | 76017 | 3866 |
| <I/σ(I)> | 10.4 | 1.8 |
| Completeness [%] | 96.9 | 82.6 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 0.993 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M ammonium acetate, 0.1 M Bis-Tris, pH 5.5, 17% PEG10000 |
