8TBQ

The crystal structure of human VISTA extra cellular domain in complex with Fab fragment of pH-selective anti-VISTA antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9998
Spacegroup name	C 1 2 1
Unit cell lengths	207.659, 39.512, 177.979
Unit cell angles	90.00, 117.12, 90.00

Refinement procedure

Resolution	158.409 - 2.593
Rwork	0.237
R-free	0.29100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.941
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	158.409	2.837
High resolution limit [Å]	2.593	2.593
Number of reflections	26185	1309
<I/σ(I)>	8.9
Completeness [%]	84.6	52.7
Redundancy	4.27	4.58
CC(1/2)	0.996	0.695

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M BIS-TRIS propane, 0.2 M NaBr, 22%PEG3350