8T8C

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, A-site aminoacyl-tRNA analog ACC-PMN, and P-site formyl-MFI-tripeptidyl-tRNA analog ACCA-IFMf at 2.60A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-11
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	209.830, 450.390, 621.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.100 - 2.600
R-factor	0.2228
Rwork	0.220
R-free	0.26920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rqc
RMSD bond length	0.004
RMSD bond angle	0.849
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	122.100	122.100	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.154	0.031	1.287
Rmeas	0.172	0.035	1.456
Total number of observations	8726049
Number of reflections	1770773	20569	130463
<I/σ(I)>	8.84	32.18	1.01
Completeness [%]	99.5	98	99.5
Redundancy	4.928	5.253	4.461
CC(1/2)	0.992	0.998	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME