8T5L

Crystal structure of STING CTD in complex with 2'3'-cGAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2023-04-26
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	88.330, 79.230, 36.590
Unit cell angles	90.00, 96.13, 90.00

Refinement procedure

Resolution	39.610 - 2.010
R-factor	0.1801
Rwork	0.178
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4emt
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.610	2.060
High resolution limit [Å]	2.010	2.010
Number of reflections	16799	1224
<I/σ(I)>	8.2
Completeness [%]	99.9
Redundancy	3.7
CC(1/2)	0.916	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 25% PEG3350