8STW
K384N HUMAN CYSTATHIONINE BETA-SYNTHASE (delta 411-551)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-02-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.976 |
Spacegroup name | P 1 |
Unit cell lengths | 89.462, 90.189, 104.224 |
Unit cell angles | 106.15, 105.49, 107.47 |
Refinement procedure
Resolution | 43.110 - 2.400 |
R-factor | 0.21206 |
Rwork | 0.210 |
R-free | 0.24295 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.666 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.110 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 100667 | 5138 |
<I/σ(I)> | 8.1 | |
Completeness [%] | 93.3 | |
Redundancy | 3.8 | |
CC(1/2) | 0.997 | 0.933 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 22 % PEG 3,350, 6 % Tacsimate pH 6.0 |