8STW

K384N HUMAN CYSTATHIONINE BETA-SYNTHASE (delta 411-551)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2022-02-18
Detector	RAYONIX MX-300
Wavelength(s)	0.976
Spacegroup name	P 1
Unit cell lengths	89.462, 90.189, 104.224
Unit cell angles	106.15, 105.49, 107.47

Refinement procedure

Resolution	43.110 - 2.400
R-factor	0.21206
Rwork	0.210
R-free	0.24295
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.666
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.110	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	100667	5138
<I/σ(I)>	8.1
Completeness [%]	93.3
Redundancy	3.8
CC(1/2)	0.997	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22 % PEG 3,350, 6 % Tacsimate pH 6.0