8SKN
Crystal structure of compound 3-bound human Dynamin-1-like protein GTPase-BSE fusion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97843 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.580, 81.270, 97.080 |
| Unit cell angles | 90.00, 101.19, 90.00 |
Refinement procedure
| Resolution | 47.660 - 2.410 |
| R-factor | 0.20819 |
| Rwork | 0.206 |
| R-free | 0.25555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4h1v |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.386 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.660 | 2.500 |
| High resolution limit [Å] | 2.410 | 2.410 |
| Rmerge | 0.175 | 0.966 |
| Rmeas | 0.189 | 1.043 |
| Rpim | 0.072 | 0.391 |
| Total number of observations | 20605 | |
| Number of reflections | 28133 | 2955 |
| <I/σ(I)> | 10.2 | 2.3 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.8 | 7 |
| CC(1/2) | 0.994 | 0.735 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.05 M potassium chloride, 0.01 M magnesium chloride, 15% w/v PEG6000 |
