8SDG
Crystal structure of SARS-CoV-2 receptor binding domain in complex with neutralizing antibody CC25.43
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-11-17 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0332 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 311.948, 83.786, 74.150 |
Unit cell angles | 90.00, 100.80, 90.00 |
Refinement procedure
Resolution | 72.840 - 2.710 |
R-factor | 0.2572 |
Rwork | 0.255 |
R-free | 0.30760 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.697 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 153.213 | 2.760 |
High resolution limit [Å] | 2.710 | 2.710 |
Number of reflections | 47774 | 2451 |
<I/σ(I)> | 7.8 | |
Completeness [%] | 93.4 | |
Redundancy | 5.2 | |
CC(1/2) | 0.995 | 0.495 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1.0 M Li-chloride, 10% PEG-6000, and 0.1 M citric acid pH 4.0 |