8S7C
Ternary Complex of Cachd1, FZD5 and LRP6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 283.697, 198.236, 218.824 |
Unit cell angles | 90.00, 128.07, 90.00 |
Refinement procedure
Resolution | 86.130 - 4.700 |
R-factor | 0.2092 |
Rwork | 0.206 |
R-free | 0.26080 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.577 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 172.259 | 5.400 |
High resolution limit [Å] | 4.700 | 4.700 |
Number of reflections | 23514 | 1177 |
<I/σ(I)> | 6.3 | |
Completeness [%] | 88.8 | |
Redundancy | 6.8 | |
CC(1/2) | 0.990 | 0.437 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 4.5 | 296 | 0.1 M Calcium Acetate 0.1 M Sodium Acetate 10% PEG 4000 pH 4.5 |