8RXB

Human UPF1 CH domain in complex with SMG6 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	87.165, 90.677, 92.291
Unit cell angles	90.00, 113.36, 90.00

Refinement procedure

Resolution	43.440 - 2.600
R-factor	0.2818
Rwork	0.281
R-free	0.29470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_5109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.440	2.630
High resolution limit [Å]	2.597	2.600
Number of reflections	75047	2523
<I/σ(I)>	6.91
Completeness [%]	93.7
Redundancy	2
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	solution (28% (w/v) PEG 3500, 0.2M Lithium sulfate and 0.1 M Tris pH 7.8)