8RVQ
20S proteasome from pre1-1
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Phase Plate | VOLTA PHASE PLATE |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.02 |
| Number of Particle | 10556408 |
| Software | cryoSPARC,SerialEM |
| Symmetry | C2 |
| CTF correction | NONE |
