8RU8
A crystal form of a human CDK2-CDK7 chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-05 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.081, 72.592, 72.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.170 - 1.510 |
| R-factor | 0.2051 |
| Rwork | 0.203 |
| R-free | 0.24080 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.180 |
| Data reduction software | X-Area |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.170 | 1.536 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.085 | |
| Rmeas | 1.492 | |
| Number of reflections | 45158 | 2202 |
| <I/σ(I)> | 10.1 | 1.1 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 6.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 12% 1,2-Propanediol .12% 1,3-Propanediol .12% 1,4-Butanediol .12M 1,6-Hexanediol .12% 1-Butanol .12% 2-Propanol 30% MPEG 500 10% PEG 20000 .1M pH=7.5 MOPS/NaHEPES |
