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8RTT

Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.

EM specimen
VitrificationYes
InstrumentFEI VITROBOT MARK IV
EM Data Collection
MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)0.01
Energy FilterGIF Bioquantum
DetectorGATAN K3 BIOQUANTUM (6k x 4k)
3D Reconstruction
MethodSINGLE PARTICLE
Resolution (Å)3.56
Number of Particle5200600
SoftwarecrYOLO,cryoSPARC,RELION,Coot,PHENIX,EPU,CTFFIND,UCSF ChimeraX
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION

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PDB entries from 2024-05-15

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