8RP8

Structure of K2 Fab in complex with human CD47 ECD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	69.415, 71.812, 78.379
Unit cell angles	109.45, 95.55, 118.90

Refinement procedure

Resolution	20.060 - 2.000
R-factor	0.182
Rwork	0.180
R-free	0.21440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.220	2.040
High resolution limit [Å]	2.000	2.000
Rmerge	0.036	0.145
Rmeas	0.043	0.171
Rpim	0.022	0.089
Number of reflections	78163	4427
<I/σ(I)>	19.2	7.5
Completeness [%]	97.4	96.8
Redundancy	3.6	3.7
CC(1/2)	0.999	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1 M sodium citrate, 20% PEG 8000