8RDS
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with spiro-isoxazol based compound 8i
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-23 |
Detector | DECTRIS EIGER2 S 16M |
Wavelength(s) | 1.0001 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.385, 59.297, 86.209 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.000 |
R-factor | 0.20883 |
Rwork | 0.207 |
R-free | 0.25337 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.860 | 48.860 | 2.120 |
High resolution limit [Å] | 2.000 | 5.960 | 2.000 |
Rmeas | 0.103 | 0.044 | 1.572 |
Number of reflections | 37772 | 1429 | 6135 |
<I/σ(I)> | 10.65 | 36.48 | 1.11 |
Completeness [%] | 100.0 | 99.8 | 99.9 |
Redundancy | 7.02 | 6.73 | 7.1 |
CC(1/2) | 0.999 | 0.998 | 0.512 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.4-0.6 M (NH4)H2PO4, ~17 mg/ml MSCI4 with 3 mM thalidomide |