8RD0

HUWE1 WWE domain in complex with compound 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000000
Spacegroup name	P 43 21 2
Unit cell lengths	62.795, 62.795, 231.755
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.270 - 1.765
R-factor	0.1975
Rwork	0.197
R-free	0.21460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.880
Data reduction software	XDS ((VERSION Jan 31)
Data scaling software	autoPROC ((Version 1.1.7))
Phasing software	PHASER
Refinement software	BUSTER (2.11.8 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.610	1.860
High resolution limit [Å]	1.765	1.765
Number of reflections	42066	2102
<I/σ(I)>	13.4
Completeness [%]	95.1
Redundancy	23
CC(1/2)	0.998	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		278	0.1 M Na Acetate pH 4.83, 2.9 M NaCl