8R9A
A soakable crystal form of human CDK7 in complex with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99987 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 144.433, 40.417, 68.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.220 - 1.710 |
| R-factor | 0.2236 |
| Rwork | 0.220 |
| R-free | 0.29390 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.305 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.220 | 1.717 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmeas | 0.173 | 1.232 |
| Number of reflections | 18453 | 969 |
| <I/σ(I)> | 7.8 | |
| Completeness [%] | 85.5 | |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | .2M Na2HPO4 20% PEG 3350 |
