8QZQ
Single particle cryo-EM co-structure of E. coli AcrB with bound BDM91531 inhibitor
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Quantum SE |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.23 |
Number of Particle | 465620 |
Software | cryoSPARC |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |