8QWN
Structure of p53 cancer mutant Y220C (hexagonal crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 45.330, 45.330, 332.640 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.300 - 1.440 |
R-factor | 0.171052924572 |
Rwork | 0.169 |
R-free | 0.20915 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.744 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.500 | 1.460 |
High resolution limit [Å] | 1.440 | 1.440 |
Rmerge | 0.046 | 1.094 |
Number of reflections | 38726 | 1789 |
<I/σ(I)> | 28 | 2.5 |
Completeness [%] | 99.4 | 94.1 |
Redundancy | 13.2 | 13.2 |
CC(1/2) | 1.000 | 0.907 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 5.5-6.0 mg/ml in 25 mM HEPES (pH 7.5), 200 mM NaCl, 0.5 mM TCEP Reservoir buffer: 20% w/v PEG 8000 (w/v), 0.1 M Tris pH 8.5, 0.2 M magnesium chloride hexahydrate. |