8QW7
Crystal Structure of compound 4 in complex with KRAS G12V C118S GDP and pVHL:ElonginC:ElonginB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2020-02-07 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.999891 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.520, 125.929, 81.056 |
Unit cell angles | 90.00, 111.61, 90.00 |
Refinement procedure
Resolution | 67.420 - 2.360 |
R-factor | 0.2476 |
Rwork | 0.246 |
R-free | 0.27260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.821 |
Data reduction software | autoPROC |
Data scaling software | autoPROC |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.420 | 2.698 |
High resolution limit [Å] | 2.360 | 2.412 |
Rpim | 0.032 | 0.453 |
Number of reflections | 30823 | 1204 |
<I/σ(I)> | 0.132 | |
Completeness [%] | 92.8 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 200 mM Lithium sulfate monohydrate, 100 mM BIS-TRIS propane pH 5.5, 25% w/v polyethylene glycol 3350 |