8QQY
Crystal structure of human Ephrin type-A receptor 2 (EPHA2) Kinase domain in complex with JG165
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.918396 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.818, 107.676, 40.636 |
| Unit cell angles | 90.00, 109.18, 90.00 |
Refinement procedure
| Resolution | 38.410 - 1.800 |
| R-factor | 0.19065 |
| Rwork | 0.189 |
| R-free | 0.22328 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.516 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.410 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 24679 | 1466 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.750 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20% PEG3350 10% ethylene glycol 0.2M sodium iodide |
