8QM7
Potential drug binding sites for translation initiation factor eIF4E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-10 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.157, 69.122, 64.435 |
| Unit cell angles | 90.00, 95.67, 90.00 |
Refinement procedure
| Resolution | 64.120 - 2.191 |
| R-factor | 0.1945 |
| Rwork | 0.192 |
| R-free | 0.24010 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | BUSTER |
| Refinement software | BUSTER (2.11.8 (16-JUL-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.120 | 2.270 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 1.880 | |
| Rmeas | 0.190 | |
| Number of reflections | 20450 | 1900 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.002M DTT 38% PEG 400 0.1M pH=8 Tris/HCl |
