8QDE

Crystal structure of a truncated human L-Lactate Dehydrogenase B protein in complex with NADH and oxamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	71.530, 85.660, 207.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.310 - 2.980
R-factor	0.2346
Rwork	0.231
R-free	0.31000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.310	3.087
High resolution limit [Å]	2.980	2.980
Rmeas	0.339	1.697
Rpim	0.092	0.455
Number of reflections	26832	2626
<I/σ(I)>	10.69	1.76
Completeness [%]	99.9	100
Redundancy	13.5	13.8
CC(1/2)	0.995	0.613

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Lithium sulfate, 0.1M sodium acetate pH 4.5, 50% (v/v) PEG 400