8Q1X

Structural analysis of PLD3 reveals insights into the mechanism of lysosomal 5' exonuclease-mediated nucleic acid degradation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98141
Spacegroup name	P 1 21 1
Unit cell lengths	60.535, 115.452, 101.335
Unit cell angles	90.00, 106.62, 90.00

Refinement procedure

Resolution	35.780 - 1.850
R-factor	0.1743
Rwork	0.174
R-free	0.19870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.989
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.550	1.970
High resolution limit [Å]	1.850	1.850
Rmeas	0.014	0.185
Number of reflections	111313	17357
<I/σ(I)>	8.13
Completeness [%]	98.4
Redundancy	4.05
CC(1/2)	0.996	0.417

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	15% PEG 4000, 0.02 M NaI, 0.9 M ammonium sulfate