8PXT

Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-30
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	92.230, 169.060, 171.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.320 - 2.250
R-factor	0.1813
Rwork	0.180
R-free	0.21020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.640
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.320	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.123
Rmeas	0.129
Rpim	0.038
Number of reflections	127867	12605
<I/σ(I)>	12.86
Completeness [%]	100.0
Redundancy	11.3
CC(1/2)	0.998	0.640

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	11% PEG8000, 0.5 M NaCl, 0.1M NaCacodylate