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8PW8

Crystal structure of the human METTL3-METTL14 in complex with a bisubstrate analogue (BA2)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2018-10-12
DetectorDECTRIS PILATUS 2M-F
Wavelength(s)1
Spacegroup nameP 32 2 1
Unit cell lengths63.780, 63.780, 225.450
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution44.510 - 2.300
R-factor0.1988
Rwork0.196
R-free0.24670
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.008
RMSD bond angle1.021
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX (1.19.1_4122)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]45.1002.440
High resolution limit [Å]2.3002.300
Number of reflections247093866
<I/σ(I)>15.74
Completeness [%]99.8
Redundancy8.1
CC(1/2)0.9900.638
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP29520% PEG 3350, 0.4M Magnesium acetate

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