8PU6
Structure of immature HTLV-1 CA-NTD from in vitro assembled MA126-CANC tubes: pooled class
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum,GIF Quantum LS |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k),GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SUBTOMOGRAM AVERAGING |
| Resolution (Å) | 3.4 |
| Software | subTOM,UCSF ChimeraX,Coot,SerialEM,CTFFIND,NOVACTF,ISOLDE,PHENIX,Warp,RELION,MATLAB,UCSF Chimera |
| Symmetry | C2 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
