8PTY
Cryo-EM structure of human Elp123 in complex with 5'-deoxyadenosine and methionine
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | FEI FALCON III (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.58 |
| Number of Particle | 264351 |
| Software | cryoSPARC,RELION,Coot,PHENIX,Topaz,EPU,UCSF ChimeraX,NAMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
