8PJE

Human Leukocyte Antigen class II allotype DR1 presenting influenza A virus haemagglutinin (HA)306-318 PKYVKQNTLKLAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	41.500, 89.180, 137.440
Unit cell angles	90.00, 90.90, 90.00

Refinement procedure

Resolution	32.810 - 1.700
R-factor	0.1724
Rwork	0.171
R-free	0.20260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.639
Data reduction software	xia2
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.810	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.083	0.884
Rmeas	0.097	1.039
Rpim	0.049	0.539
Number of reflections	109380	10898
<I/σ(I)>	9.48	1.36
Completeness [%]	99.5	99.55
Redundancy	3.7	3.6
CC(1/2)	0.997	0.545

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.1 M HEPES pH 7.0, 25 % PEG8000, 15 % Glycerol